Mouse Naïve CD4+ T Cell Isolation Kit

The Mouse Naïve CD4+ T Cell Isolation Kit delivers a high purity population of murine Naïve CD4+ T cells that are ready for downstream use. The BACSTM Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of Naïve CD4+ T cells during enrichment., , Mechanism of Action: The Mouse Naïve CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich Naïve CD4+ T cells. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD49b, CD105, Gr1, Ter119, TCRgammadelta., , How it works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACSTM Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. Naïve CD4+ T cells, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched Naïve CD4+ T cells healthy and ready for downstream use., , . Cell Type: Na?ve CD4+ T cells, , . Sample Species and Source: Mouse spleen, , . Cell Separation Method: Negative selection, , . Capacity: 1 billion cells, , Kit Includes:, , . BACSTM Streptavidin Microbubbles, , . Mouse Na?ve CD4+ T Cell Biotin Antibody Cocktail, , . Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA), , . 5 ml Tubes