Mouse B Cell Isolation Kit

The Mouse B Cell Isolation Kit delivers a high purity population of murine B cells that are ready for downstream use. The BACSTM Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of B cells during enrichment., , Mechanism of Action: The Mouse B Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich B Cells. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD3, CD4, CD8a, CD11b, CD11c, CD49b, CD90. CD105, Gr1, Ter119., , How it works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACSTM Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. B cells, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched B cells healthy and ready for downstream use., , . Cell Type: B cells, , . Sample Species and Source: Mouse spleen, , . Cell Separation Method: Negative selection, , . Capacity: 1 billion cells, , Kit Includes:, , . BACSTM Streptavidin Microbubbles, , . Mouse B Cell Biotin Antibody Cocktail, , . Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA), , . 5 ml Tubes