GST pull-downn Kit (Agarose)

Coupled ligand: GSH. Beads: Sepharose 4B agarose with average particle size of 50 µm. Binding capacity: 1.2mg protein /ml agarose. Storage buffer: PBS with preservative and 50% glycerol, pH7.2. Storage conditions: -20? for 12 months. Background: The GST Pull-Down experiment is based on the reversible specific binding between glutathione-S-transferase (GST) and glutathione (GSH). The corresponding target protein is isolated and purified by GST fused bait protein. The bait protein fuses with GST, and the GST-bait fusion protein binds to GSH-agarose gel complex. If the target protein interacts with the bait protein in the environment, the "agarose gel-GSH-GST-bait protein-target protein" complex will be formed, and then the target protein interacting with the bait protein can be separated and detected. This product is made of high-quality reduced glutathione (GSH) and agarose gel by covalent binding. It has the characteristics of high binding capacity of protein, fast and convenient operation, strong specificity and specificity, and can specifically combine with proteins containing GST tags in cells or microbial lysates.