EasySort(TM) Human Naïve CD8+T Cell Isolation Kit

Introduction EasySort(TM) Human Naïve CD8+ T Cell Isolation Kit can help researcher isolate high purity human Naïve CD8+T cells with simple experimental procedure. The kit is suitable for isolation of Naïve CD8+T cells from fresh human PBMC or frozen PBMC, and the isolated Naïve CD8+T cells can be directly used for downstream applications. Intende use The kit is suitable for isolation of Naïve CD8+T cells from fresh human PBMC or frozen PBMC. Isolation Technology Negative Isolation Target Species Human Cell Type Human Naïve CD8+T Cell Sample Type human PBMC Starting Material Cell Number 1 x 10^7 cells per assay Protein function: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells [The UniProt Consortium]