EasySort(TM) Human Naïve CD4+T Cell Isolation Kit

Introduction EasySort(TM) Human Naïve CD4+T cell isolation kit is a product that can isolate high purity human Naïve CD4+T cells quickly and easily. The kit is suitable for isolation of Naïve CD4+T cells from fresh human PBMC or frozen PBMC, and the isolated cells can be directly used for downstream applications. Intende use The kit is suitable for isolation of Naïve CD4+T cells from fresh human PBMC or frozen PBMC. Isolation Technology Negative Isolation Target Species Human Cell Type Human Naïve CD4+T Cell Sample Type human PBMC Starting Material Cell Number 1 x 10^7 cells per assay Protein function: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:12089508, PubMed:16331979, PubMed:2214026, PubMed:9641677). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607) [The UniProt Consortium]