Tissue Cryopreservation Reagent Kit (MEDY)

The components of this kit can be combined to produce the small molecule cocktail MEDY. When mixed with the appropriate cell culture media, MEDY enables the cryopreservation of human brain tissue samples and neural organoids that retain pathological features after thawing. Following a three-week pretreatment outlined by Xue et al., this optimized cocktail mixed with week four maturation media enables reliable storage of diverse organoids and living brain tissues. Each component in this kit is provided separately, and instructions are included to reconstitute these reagents for direct dilution into week four cell culture media. The kit is intended to supplement 100 ml of final media when following the supplied instructions. Some reagents may be provided in excess and may be properly disposed of after cell media preparation. Prior to use, reagents must be stored as indicated at -20°C.