Stem Cell Expansion Kit (SCB)

The components of this kit can be combined to produce the small molecule cocktail SCB. When mixed with the appropriate cell culture media, SCB promotes cell expansion and increases the clonogenic potential of genetically stable hematopoietic stem cells (HSCs). SB-431542 is a potent and selective inhibitor of activin-like kinase 5 (ALK5), also known as TGF-beta receptor type 1 (TGF-betaRI), that increases the proliferation of HSCs. CHIR99021 is an inhibitor of glycogen synthase kinase 3 (GSK-3) that regulates Wnt/beta-catenin signaling and plays an important role in the differentiation of HSCs. bpV(pic) is a selective PTEN inhibitor that increases HSC proliferation and survival.5, , Each component in this kit is provided separately, and instructions are included to reconstitute and mix these reagents for direct dilution into cell culture media. The kit is intended to supplement up to 2 L of culture media. Some reagents may be provided in excess and may be properly disposed of after cell media preparation. Prior to use, reagents must be stored as indicated at -20°C.