ReadiLink(TM) iFluor(R) 555 FISH Fluorescence Imaging Kit

Fluorescence in situ hybridization (FISH) technology is an effective tool for detecting specific nucleic acid targets in a biological specimen. Detection of a nucleic acid target in situ is achieved through the hybridization of a fluorescent dye-labeled nucleic acid probe of complementary sequence to the specimen. The Readilink(TM) iFluor(R) 555 FISH fluorescence imaging kit is a convenient tool for labeling a target DNA using an iFluor(R) 555 labeled FISH probe via in situ hybridization. The kit provides Taq DNA polymerase enzyme which incorporates iFluor(R) 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR). Our proprietary iFluor(R) dyes are brighter and more photostable than traditional fluorescent labels, providing the desired resolution and signal. Solvent: Water. Correction Factor (260 nm): 0.23. Correction Factor (280 nm): 0.14. Extinction coefficient (cm^-1 M^-1): 100000 (aqueous buffer (pH 7.2)). Excitation (nm): 557. Emission (nm): 570. Quantum yield: 0.64 (aqueous buffer (pH 7.2)).