RAW 264.7 Polarized M1 Macrophage Induction and Identification Kit

Macrophages, as heterogeneous and diverse immune cells with unique morphological and functional properties, are involved in inflammatory responses, autoimmunity, and injury repair. Macrophage polarization plays a crucial role in eliminating pathogens or maintaining tissue homeostasis. In recent years, it has been widely accepted that the development of various immune-related diseases is influenced by macrophage polarization. In a complex and variable microenvironment, macrophages can be transformed into different phenotypes. In general, macrophages have at least two different polarizations: M1-type macrophages and M2-type macrophages. Macrophages are transformed into M1-type macrophages when stimulated by substances such as lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). These macrophages produce high levels of nitric oxide synthase (iNOS), interleukin-6 (IL-6), and tumor necrosis factor-a (TNF-a), which promotes an inflammatory response.RAW 264.7 (mouse monocyte macrophage leukemia cells) is one of the most commonly used in vitro models for osteoclast and inflammation studies, and is widely used to study rheumatoid arthritis, osteoporosis, osteolysis, periodontitis and other skeletal diseases.This kit provides a complete set of differentiation and identification protocols for RAW 264.7 polarized to M1 macrophages,which provides stable and reliable support for the differentiation and identification of RAW 264.7 polarized to M1 macrophages.