Isotyping Kit: Rapid Mouse Monoclonal Isotyping Kit IsoMax(TM) - 5 pack

Rockland Immunochemicals' IsoMax(TM) Rapid Mouse Monoclonal Isotyping Kit is a rapid (5 minute) lateral flow assay with sensitivity equal to ELISA for monoclonal antibody class and subclass determination. The assay is designed to test antibody present in tissue culture supernatant or mouse ascites fluid. This kit contains 5 packs of 2 cassettes each sufficient for testing up to 5 samples. Please read the entire product insert prior to use. Use Rockland Immunochemicals' IsoMax(TM) Rapid Mouse Monoclonal Isotyping Kit for determining the class and subclass of a monoclonal antibody. Isotype determination is a prerequisite for selecting the best immunoglobulin purification method. For example, IgA and IgM are often best purified by size (gel filtration) or on immunoaffinity support columns, whereas IgG2a and IgG2b can be purified by protein A chromatography at a pH of 7 to 8. IgG1 binds best to protein A at a pH of 8 to 9. Isotype determination is also required if an antibody is to be digested by pepsin or papain as each isotype shows varying sensitivity to enzymatic digestion. Please call technical service for further assistance.