Human TNF-alpha TR-FRET Detection Kit (10000 tests)

Tumor Necrosis Factor Alpha (TNF alpha) is a pro-inflammatory cytokine mainly produced by macrophages and monocytes. It can directly kill tumor cells without significant toxicity to normal cells. TNF alpha assembles intracellularly to form a non-covalently linked homotrimer and is expressed on the cell surface. It plays a role in normal inflammatory and immune responses by coordinating tissue homeostasis through the regulation of other cytokines' production, cell survival, and death. This reagent kit utilizes a sandwich method to detect TNF alpha levels. The kit contains TNF alpha standard and two monoclonal antibodies which can specific bind to TNF alpha, one coupled with Eu (donor, Anti-TNF alpha-Eu) and the other coupled with A3 (acceptor, Anti-TNF alpha-A3). When two antibodies bind to TNF alpha simultaneously, Anti-TNF alpha-Eu and Anti-TNF alpha-A3 come close together, leading to fluorescence resonance energy transfer (FRET). Use 320/340 nm excitation light to excite the fluorescent donor, which emits 620 nm light. The 620 nm light excites the fluorescent receptor, which emits 665 nm light. The TNF alpha concentration in the sample is proportional to the FRET signal (ratio of fluorescence intensity at 665 nm to 620 nm).