Human IL-2 TR-FRET Detection Kit (500 tests)

Interleukin-2 (IL2) is a key immunoregulatory cytokine primarily produced by activated T cells. It plays crucial roles in promoting the proliferation of T lymphocytes and natural killer (NK) cells, stimulating the differentiation and proliferation of B cells, and enhancing antibody production. IL2 is essential in immune responses, immune regulation, and anti-tumor immunity within the body. This reagent kit utilizes a sandwich method to detect IL2 levels. The kit contains IL2 Standard and two monoclonal antibodies specific to IL2, one coupled with Eu (donor, Anti-IL2-Eu) and the other coupled with A3 (acceptor, Anti-IL2-A3). When both antibodies bind to IL2 simultaneously, the proximity of Anti-IL2-Eu and Anti-IL2-A3 allows for fluorescence resonance energy transfer (FRET) to occur. Use 320/340 nm excitation light to excite the fluorescent donor, which emits 620 nm light. The 620 nm light excites the fluorescent receptor, which emits 665 nm light. The IL2 concentration in the sample is proportional to the FRET signal (ratio of fluorescence intensity at 665 nm to 620 nm).