Human IFN-gamma TR-FRET Detection Kit (500 tests)

Interferon-gamma (IFN gamma) is a pro-inflammatory cytokine primarily produced by T cells and natural killer (NK) cells, playing a crucial role in antiviral defense mechanisms within the body. IFN-gamma assembles into soluble dimers intracellularly and is secreted into the extracellular space. It is involved in antiviral defense, activation of macrophages, enhancement of neutrophil and monocyte function, and upregulation of MHC-I and MHC-II expression in cells. This reagent kit utilizes a sandwich method to detect IFN gamma levels. The kit contains IFN gamma Standard and two monoclonal antibodies specific to IFN gamma, one coupled with Eu (donor, Anti-IFN gamma-Eu) and the other coupled with A3 (acceptor, Anti-IFN gamma-A3). When both antibodies bind to IFN gamma simultaneously, the proximity of Anti-IFN gamma-Eu and Anti-IFN gamma-A3 allows for fluorescence resonance energy transfer (FRET) to occur. Use 320/340 nm excitation light to excite the fluorescent donor, which emits 620 nm light. The 620 nm light excites the fluorescent receptor, which emits 665 nm light. The IFN gamma concentration in the sample is proportional to the FRET signal (ratio of fluorescence intensity at 665 nm to 620 nm).