Hepatocyte Maintenance Reagent Kit

The components of this kit, when combined with an appropriate cell culture media, enable maintenance of primary hepatocytes as terminally differentiated cells in long-term culture. Each component in this kit is provided separately, and instructions are included to reconstitute these reagents for direct dilution into cell culture media. The kit is intended to supplement up to 4 L of culture media when using the indicated concentrations. Some reagents may be provided in excess and may be properly disposed of after cell media preparation. Prior to use, reagents must be stored as indicated at -20°C.