Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

Detection Principle: Dihydroxyacetone phosphate (DHAP) is an intermediate product present in the glycolysis and gluconeogenesis processes of living organisms. Most animals, due to the absence of the glyoxylate cycle, cannot convert fatty acids into glucose like plants. They can only convert the glycerol in fats into glucose through DHAP or enter the glycolysis pathway. Detection principle: The products formed after a series of enzymatic reactions of DHAP can cause the fluorescent probe to emit fluorescence. The fluorescence value can be detected using a fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm to calculate the content of DHAP.