COVID-19 (SARS-CoV-2) & Influenza A/B Multiplex QPCR Detection Kit

This product is a multiplex reverse transcription quantitative real-time PCR (RT-qPCR) kit based on Taqman probe method. The 3' end of each detection probe is labeled with a quenching group, and the 5' end is labeled with a different reporter group. When the probe is intact, since the quenching group is close to the reporter group, the fluorescence emitted by the reporter group is absorbed by the quenching group, and no fluorescence signals are detected. When the primer is extended, the fluorescent probe bound to the template is cleaved by Taq enzyme (5'->3' exonuclease activity), the reporter group is separated from the quenching group, and fluorescence signals are generated. The fluorescent quantitative PCR instrument can automatically draw a real-time amplification curve based on the detected fluorescence signals, so as to realize the qualitative detection at the nucleic acid level. The multiplex RT-qPCR detection system also includes a pair of primers and probe for internal control, which can be used to monitor the sample collection, sample handling and RT-qPCR process to avoid a false-negative result.