Cathepsin D (CatD) Activity Fluorometric Assay Kit

Cathepsin D (CatD) is an important intracellular aspartic acid protease and is widely present in the biological world. Cathepsin D has multiple biological functions and is involved in intracellular protein degradation, apoptosis and autophagy, etc. The detection principle of this kit: CatD hydrolyzes the substrate to generate the chromogenic substance. The activity of cathepsin D in the sample is calculated by measuring the changes in fluorescence values at the excitation wavelength of 328 nm and the emission wavelength of 393 nm. The recommended dilution factor for different samples is as follows (for reference only): Mouse serum: 1-2, Mouse plasma: 1, 10% Rat pancreas tissue homogenate: 1-2, 10% Rat spleen tissue homogenate: 1, 10% Rat small intestine tissue homogenate: 1-2, 10% Rat brain tissue homogenate: 1, 10% Rat stomach tissue homogenate: 1, 10% Rat kidney tissue homogenate: 2-4, 10% Rat liver tissue homogenate: 2-8, 1-10^6 Hela cells: 1, 1-10^6 HL-60 cells: 1, 1-10^6 293T cells: 1. The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.