Other Toolkits

Mitochondrial stress refers to the stress state that occurs when mitochondria are damaged or overloaded under the influence of external or internal factors (such as oxidative stress, nutritional deficiency, toxins, etc.). This kind of stress can affect the energy production of mitochondria, leading to insufficient...

The senescence-associated beta-galactosidase (SA-beta-gal) is a key biomarker of cellular senescence. Studies have shown that the activity of this enzyme significantly increases in senescent cells and is related to abnormal lysosomal function. It is commonly used to detect the state of cellular senescence and study...

Detection Principle: Hydrogen Sulfide (H2S), as a key signal transduction gas molecule in cells and organisms, is involved in numerous physiological and pathological processes such as vascular relaxation and nerve transmission. The detection of intracellular H2S can deeply explore its specific mechanism and role in...

Detection Principle: Dihydroxyacetone phosphate (DHAP) is an intermediate product present in the glycolysis and gluconeogenesis processes of living organisms. Most animals, due to the absence of the glyoxylate cycle, cannot convert fatty acids into glucose like plants. They can only convert the glycerol in fats into...

Detection Principle: Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the fluuorescence intensity of the light signal is proportional to the ATP content.

Detection Principle: Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content.

The Fenton reaction is the most common chemical reaction that generates hydroxyl radicals (OH·). The amount of hydrogen peroxide is directly proportional to the amount of OH· produced by the Fenton reaction. After the electron accepter is given, adding griess reagent to produce the red substance, which is directly...

The Total Amino Acids (T-AA) Colorimetric Assay Kit (E-BC-K055-S) is designed to measure total amino acid content using a colorimetric method. The detection principle is based on copper ions forming blue-green complexes with various amino acids, where the color intensity at 650 nm is directly proportional to the...

Biotin is an important biological molecule that is widely used to label antibodies or other proteins in biotinylated reactions. The detection principle: Biotin can compete with avidin for binding to the dye, causing a decrease in the absorbance value at 500 nm, calculate the content of biotin.

Oxalic, also known as ethanedioic acid, is a simple organic acid in plants and plays important physiological roles. Oxalic participates in the regulation of Ca2+, pH and osmotic pressure within cells, as well as the defense responses of plant pathogens and the alleviation of aluminum toxicity. Although Oxalic has...

6-phosphogluconate dehydrogenase (6-PGDH) is the rate-limiting enzyme of the pentose phosphate pathway, which is responsible for the production of NADPH, energy balance, growth rate, and cell viability. 6-PGDH is also associated with a variety of tumor signaling pathways, including epidermal growth factor signaling...

Detection Principle: Sucrose synthase-I (SS- I) catalyzes the substrate reaction to generate reducing sugar. The reducing sugar can show color changes when reacting with the chromogenic agent. There is a characteristic absorption peak at 540 nm. The activity of the enzyme is directly proportional to the absorbance.