Other Toolkits

This kit can be used to extract mitochondrial from cells. The differential centrifugation method is the most commonly used method for separating cell mitochondria. Usually, the first step is to prepare the cell homogenate, then perform low-speed centrifugation to remove the cell nucleus, cell debris, and undamaged...

Xanthine and hypoxanthine are important alkaloid purines that can be used to treat symptoms such as asthma relief and bronchodilation. Hypoxanthine can be oxidized by xanthine oxidase to form xanthine. Xanthine is catalyzed by xanthine oxidase to form uric acid. When xanthine oxidase is deficient, xanthine...

Histone deacetylases (HDAC) are a type of protease that play an important role in the structural modification of chromosomes and the regulation of gene expression. Earlier studies have shown that HDAC has a close relationship with tumors. The overexpression of HDAC can lead to various types of cancer as well as...

Cathepsin D (CatD) is an important intracellular aspartic acid protease and is widely present in the biological world. Cathepsin D has multiple biological functions and is involved in intracellular protein degradation, apoptosis and autophagy, etc. The detection principle of this kit: CatD hydrolyzes the substrate...

Mitochondria are the energy centers of cells and play a very important role in the processes of apoptosis and necrotic cell death. Mitochondrial permeability transition pore (mPTP), also known as mitochondrial megachannel (MMC), It is a non-specific channel composed of the inner and outer membranes of mitochondria,...

Mitochondrial stress refers to the stress state that occurs when mitochondria are damaged or overloaded under the influence of external or internal factors (such as oxidative stress, nutritional deficiency, toxins, etc.). This kind of stress can affect the energy production of mitochondria, leading to insufficient...

The senescence-associated beta-galactosidase (SA-beta-gal) is a key biomarker of cellular senescence. Studies have shown that the activity of this enzyme significantly increases in senescent cells and is related to abnormal lysosomal function. It is commonly used to detect the state of cellular senescence and study...

Detection Principle: Hydrogen Sulfide (H2S), as a key signal transduction gas molecule in cells and organisms, is involved in numerous physiological and pathological processes such as vascular relaxation and nerve transmission. The detection of intracellular H2S can deeply explore its specific mechanism and role in...

Detection Principle: Dihydroxyacetone phosphate (DHAP) is an intermediate product present in the glycolysis and gluconeogenesis processes of living organisms. Most animals, due to the absence of the glyoxylate cycle, cannot convert fatty acids into glucose like plants. They can only convert the glycerol in fats into...

Detection Principle: Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the fluuorescence intensity of the light signal is proportional to the ATP content.

The Total Amino Acids (T-AA) Colorimetric Assay Kit (E-BC-K055-S) is designed to measure total amino acid content using a colorimetric method. The detection principle is based on copper ions forming blue-green complexes with various amino acids, where the color intensity at 650 nm is directly proportional to the...

Biotin is an important biological molecule that is widely used to label antibodies or other proteins in biotinylated reactions. The detection principle: Biotin can compete with avidin for binding to the dye, causing a decrease in the absorbance value at 500 nm, calculate the content of biotin.