Other Toolkits

Detection Principle: Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content.

The Fenton reaction is the most common chemical reaction that generates hydroxyl radicals (OH·). The amount of hydrogen peroxide is directly proportional to the amount of OH· produced by the Fenton reaction. After the electron accepter is given, adding griess reagent to produce the red substance, which is directly...

The Total Amino Acids (T-AA) Colorimetric Assay Kit (E-BC-K055-S) is designed to measure total amino acid content using a colorimetric method. The detection principle is based on copper ions forming blue-green complexes with various amino acids, where the color intensity at 650 nm is directly proportional to the...

Biotin is an important biological molecule that is widely used to label antibodies or other proteins in biotinylated reactions. The detection principle: Biotin can compete with avidin for binding to the dye, causing a decrease in the absorbance value at 500 nm, calculate the content of biotin.

Oxalic, also known as ethanedioic acid, is a simple organic acid in plants and plays important physiological roles. Oxalic participates in the regulation of Ca2+, pH and osmotic pressure within cells, as well as the defense responses of plant pathogens and the alleviation of aluminum toxicity. Although Oxalic has...

Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color...

, Detection Principle , Revertose are sugars with reducing properties. In sugars, both monosaccharides containing free aldehyde or ketone groups and disaccharides containing free aldehyde groups are reductive. Revertose mainly include glucose, fructose, galactose, lactose, maltose, etc. Revertose are reduced to...

Phosphoglucose Isomerase (PGI) is an important enzyme that can catalyze the interconversion between glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P), and is involved in glycolysis and the pentose phosphate pathway. These processes help cells break down glucose for energy and generate key substances in other...

Acyl-CoA synthase (ACS) is an important enzyme that is widely present in living organisms and participates in the metabolic process of fatty acids. It is an enzyme necessary for lipid synthesis, fatty acid catabolism and membrane remodeling, and can also function as transcriptional activator, allosteric inhibitor or...

Sialic acid (SA) is widely present in animals and microorganisms, usually in the form of oligosaccharides, glycolipids or glycoproteins, and is an important component of cell membranes. SA is located at the ends of cell membrane glycoproteins and glycolipids, and participates in various physiological functions such...

Detection Principle: Malondialdehyde (MDA) in the degradation products of peroxidized lipids can react with thiobarbituric acid (TBA) under high temperature and acidic conditions to produce the red-brown product 3,5,5 '-trimethyloxazole-2, 4-dione (trimethine), and this substance has the maximum absorption peak at...

NADPH Oxidase (NAO) is a membrane protein mainly found in mammalian neutrophils, plant cells and filamentous fungal cells, and was initially discovered in phagocytic cell membranes. NAO can oxidize NADPH to NAD+ while generating superoxide anions, and it is one of the main sources of reactive oxygen species (ROS) in...