NZYSupreme Mutagenesis kit

NZYSupreme Mutagenesis kit provides a simple and highly efficient method to generate point mutations or to delete/insert single or multiple nucleotides in any type of plasmid DNA using PCR. This mutagenesis kit was recently developed to reduce the working timeand to increase the efficiency of DNA editing. The system requires the provision of two synthetic partially complementary oligonucleotide primers containing the desired mutation. Incorporation of these mutated oligonucleotide primers with Supreme NZYProof DNA polymerase, an engineered highly accurate polymerase, generates a mutated plasmid containing staggered nicks, which resists to Dpn I digestion (as the synthetic DNA is notmethylated). After Dpn I treatment, the resulting mutated plasmidis recovered through transformation of NZYStar competent cells. For a schematic presentation of the mutagenesis protocol, see Figure 1. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template for the mutagenesis reaction. Components: * Supreme NZYProof 2x Colourless Master Mix * Dpn I (10 U/µL) * Control plasmid * Mutagenesis Control primer mix * Competent Cells Control Plasmid (0.1 ng/µL) (*)(*) only provided in MB44702 kit. Shipping Conditions: Dry Ice. Storage Conditions: Competent cells: -85°C to -65°C-Other components: -30°C to -15°C. Application: Site-directed mutagenesis. Features: Mutagenesis of plasmids <15 kb, Fast. Product Category: DNA Cloning & Mutagenesis