Anti-Ubiquitin Remnant (K-epsilon-GG) Immunoaffinity Sorbent

Ubiquitin remnant (K-epsilon-GG) is a diglycyl-lysine motif found in proteins subject to ubiquitination, a process for tagging proteins for proteasomal degradation that is disrupted in certain diseases. During ubiquitination, ubiquitin is covalently bound to the epsilon-NH2 in lysine residues of the target protein via the C-terminal carboxyl group of ubiquitin, which is preceded by arginine-glycine-glycine (-RGG) residues. Ubiquitinated proteins can be detected, and enriched for, via antibodies toward the K-epsilon-GG motif resulting from this covalent bond. Cayman's Ubiquitin Remnant (K-epsilon-GG) Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of ubiquitin remnant (K-epsilon-GG) motif-containing proteins from biological samples. This is an effective way to concentrate ubiquitin remnant (K-epsilon-GG) motif-containing proteins for subsequent detection and analysis by LC-MS/MS. The ubiquitin remnant (K-epsilon-GG) affinity sorbent consists of Cayman's Diglycyl-Lysine Monoclonal Antibody (Clone GX41) (Cay-21096) coupled to CNBr-activated Sepharose 4B.Immunogen: Diglycine-modified histone peptide. Clone Designation: GX41. Formulation: 400 µl of CNBr-activated Sepharose 4B coupled to Diglycyl-Lysine Monoclonal Antibody (Clone GX41) (Item No. 21096). Storage Buffer: PBS, pH 7.2, with 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: IP (LC/MS certified). Cross Reactivity: (+) Diglycyl-lysine moieties.