Anti-MR1 Immunoaffinity Sorbent

Major histocompatibility complex (MHC) class I-related gene protein (MR1) is a non-polymorphic MHC class Ib antigen-presenting cell surface molecule that is required for T cell receptor-mediated activation of mucosal-associated invariant T (MAIT) cells. It is composed of alpha1 and alpha2 domains, which form an antigen-binding pocket, and an alpha3 domain that interacts with beta2-microglobulin. MR1 mRNA is ubiquitously expressed and, following translation, MR1 protein is localized to the endoplasmic reticulum in a partially folded state. Upon binding of a riboflavin-derived microbial antigen, MR1 undergoes a conformational change and translocates to the cell surface where it induces MAIT cell activation via an interaction with the MAIT cell T cell receptor and activates various immunomodulatory effects, including cytokine release, initiation of adaptive immune responses, and promotion of tissue repair. Cayman's MR1 Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of MR1 protein from biological samples. This is an effective way to concentrate MR1 protein for subsequent detection by a different MR1 antibody by immunoprecipitation (IP) (LC/MS certified) and Western blot (WB). The MR1 affinity sorbent consists of Cayman's MR1 Monoclonal Antibody (Clone 2H7) (Cay-34257) coupled to CNBr-activated Sepharose 4B.Synonyms: Class I Histocompatibility Antigen-like Protein, Major Histocompatibility Complex Class I-related Gene Protein, MHC Class I-related Gene Protein. Immunogen: Recombinant human MR1 (aa 22-292) protein. Clone Designation: 2H7. Formulation: 400 µl of CNBr-activated Sepharose 4B coupled to a human MR1 monoclonal antibody (Clone 2H7) (Item No. 34257). Storage Buffer: PBS, pH 7.2, with 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: IP, WB, LC-MS. Cross Reactivity: (+) MR1. UniProt Accession #: Q95460.