ReadiUse(TM) Preactivated APC Maleimide [Activated Allophycocyanin]

Extinction coefficient (cm -1 M -1): 700000. Excitation (nm): 651. Emission (nm): 660. ReadiUse(TM) Preactivated APC Maleimide is prepared by reacting highly purified allophycocyanin (APC) with SMCC. The NHS group of SMCC reacts with the lysine groups of APC, leaving maleimide groups available to react with free sulfhydryl groups on proteins to be conjugated. ReadiUse(TM) Preactivated APC Maleimide is highly purified to completely remove the unreacted SMCC, and lyophilized to powder. It is ready to use and will conjugate without further preparation upon mixing with sulfhydryl-containing target molecules such as reduced IgGs. The activated allophycocyanin can be easily conjugated to antibodies and other proteins without the use of added chemical crosslinking agents. These highly purified phycobiliproteins maintain their spectral characteristics of APC.