ReadiLink(TM) Rapid iFluor(R) 647 Antibody Labeling Kit *Production Scale*

Correction Factor (260 nm): 0.03. Correction Factor (280 nm): 0.03. Correction Factor (656 nm): 0.0793. Extinction coefficient (cm^-1 M^-1): 250000 (aqueous buffer (pH 7.2)). Excitation (nm): 656 Emission (nm): 670 Quantum yield: 0.25 (aqueous buffer (pH 7.2)). ReadiLink(TM) Rapid Antibody Labeling Kits, designed for production scale, provide a convenient and efficient method for labeling large volumes of antibodies with our superior iFluor(R) dyes, XFD dyes (equivalent to Alexa Fluor(R)), and various other labels. These kits utilize reactive fluorophores modified with succinimidyl ester (SE) functional groups, which selectively bind to primary amines on proteins, resulting in remarkably bright and photostable conjugates. Every kit contains all the necessary components for three distinct labeling reactions and features a user-friendly, pre-packed spin column for efficient dye removal, maximizing conjugate yield. Each vial of iFluor(R) 647 SE dye provided in the kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it is important to remove stabilizing proteins like BSA from the sample and refrain from using amine-rich buffers like Tris, which might disrupt the labeling process. iFluor(R) 647 SE is a bright, far-red fluorescent dye with excitation and emission maxima of ~656 nm and ~670 nm, making it an excellent alternative to Cy5 and Alexa Fluor(R) 647 (Alexa Fluor(R) is the trademark of Invitrogen). The high fluorescence quantum yield and photostability of iFluor(R) 647 labeled antibodies allow for the direct imaging of low-abundance targets with greater sensitivity.