ReadiCleave(TM) Cy5 NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity. Many biological molecules are labeled with fluorescent tags for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis. AAT Bioquest's ReadiCleave(TM) linkers enable fluorescent tags conjugated to a bioligocal target from which the added fluorescent tag can be removed when needed. This ReadiCleave(TM) Cy5 contains an azidomethyl linker that can be cleaved with TCEP to remove the Cy5 fluorophore from the target molecule. The cleavage can be carried out by adding 100 mM TCEP solution (pH 9.0), and incubating at 65 °C for 20-30 min.