Buccutite(TM) Rapid PE-iFluor(R) 710 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling

The Buccutite(TM) Rapid PE-iFluor(R) 710 Tandem Antibody Labeling Kit offers a highly efficient and reproducible method for small-scale conjugation of antibodies with PE-iFluor(R) 710. Utilizing the advanced Buccutite(TM) crosslinking platform, this kit significantly simplifies the labeling workflow compared to conventional strategies such as SMCC-mediated crosslinking. The streamlined two-step protocol allows for the rapid conjugation of antibodies or proteins to PE-iFluor(R) 710 in under two hours, with minimal hands-on time. Each kit includes all reagents necessary for two labeling reactions, with each reaction optimized to conjugate 100 µg of purified antibody or protein using Buccutite(TM) FOL-Activated PE-iFluor(R) 710. For optimal performance, the removal of stabilizing proteins (e.g., BSA) and avoidance of amine-containing buffers such as Tris are critical, as these components can interfere with the conjugation chemistry. PE-iFluor(R) 710 is a tandem fluorophore with excitation and emission maxima at ~565 nm and ~747 nm, respectively, offering a substantial Stokes shift and high fluorescence intensity. These properties make it particularly well-suited for applications such as multicolor flow cytometry, spectral flow cytometry, and other fluorescence-based immunoassays requiring high sensitivity. However, due to its limited photostability, it is not recommended for applications involving prolonged light exposure. This kit enables direct labeling of primary antibodies, eliminating the need for secondary antibody-based detection systems. The resulting conjugates reduce experimental complexity and enhance assay sensitivity and reproducibility.