Buccutite(TM) Rapid APC-iFluor(R) 750 Tandem Antibody Labeling Kit *Microscale Optimized for Labelin

Extinction coefficient (cm^-1 M^-1): 700000 Excitation (nm): 651 Emission (nm): 791. APC-iFluor(R) 750 Tandem is an excellent replacement for APC-Cy7 and APC-Alexa Fluor(R) 750 Tandem since they have almost identical spectra. On some antibodies, our APC-iFluor(R) 750 Tandem is much brighter than APC-Cy7, APC-H7, and APC-Alexa Fluor(R) 750 Tandem with a higher stain index. AAT Bioquest offers this Buccutite(TM) rapid labeling kit to facilitate the APC-iFluor(R) 750 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite(TM) APC-iFluor(R) 750 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC-iFluor(R) 750 tandem. The kit includes a preactivated APC-iFluor(R) 750 tandem and reaction buffer. The entire process only requires two simple mixings without further purification required. The conjugated antibody can be used in WB, ELISA, and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 25 µg of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-iFluor(R) 750 tandem to facilitate the APC-iFluor(R) 750 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-iFluor(R) 750 tandem is ready to conjugate, giving a much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-iFluor(R) 750 tandem is conjugated to a protein via its amino group that is abundant in proteins, while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.