Conjugation Kits

Protein-protein conjugations are commonly performed with a bifunctional linker (such as SMCC) having different reactivity at each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) of lysine and N-terminus, and the other end reacts (via maleimide) with the...

The SiMPLe CHC Antibody Labeling Kit leverages Sortase Mediated Protein Ligation to specifically label recombinant antibodies containing the sequence LPXTG at the C-terminus of the heavy chain (CHC). A diverse number of poly-Glycine (G)n molecules and labels (e.g. fluorophores, biotin, enzymes, peptides, etc.) are...

Ex: 651 nm. Em: 662 nm. Soluble in: Water. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and...

For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile...

In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies...

Molecular weight: 643.40. Excitation (nm): 499. Emission (nm): 520. Extinction coefficient (cm-1 M-1): 73000. Quantum yield: 0.92. Correction factor (280 nm): 0.11. XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor(R) 488 (Alexa Fluor(R) is the trademark of ThermoFisher)....

Our Cell Explorer(TM) fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to...

Tetramethylrhodamine-5-maleimide is the purified single isomer of tetramethylrhodamine-5 (and 6)-maleimide mixed isomers. It is widely used for thiol modifications of peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since...

Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red(R), a trademark of Molecular Probes). This sulforhodamine 101 sodium salt is more water-soluble than the neutral...

Solvent: DMSO. Excitation (nm): 649 Emission (nm): 664 Extinction coefficient (cm-1 M-1): 250000. Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was...

Solvent: DMSO. Excitation (nm): 656 Emission (nm): 670 Extinction coefficient (cm-1 M-1): 250000 Quantum yield: 0.25. Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2...

ReadiLink(TM) xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Preactivated iFluor(TM) 555 used in this ReadiLink(TM) kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for...