Conjugation Kits

Ex: 358 nm. Em: 450 nm. Soluble in: DMSO. DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP...

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 555 dyes have...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor(TM) 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with...

Ex: 555 nm. Em: 575 nm. The Cell Navigator(TM) Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. It may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for...

Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the...

Readilink(TM) protein labeling technology is the most robust and convenient tool for preparing fluorescent antibody conjugates used for fluorescence imaging and flow cytometry applications. ReadiLink(TM) Rapid AF488 Antibody Labeling Kit provides the most convenient tool for making Alexa Fluor(R) 488-labeled...

Ex: 543 nm. Em: 571 nm. Soluble in: DMSO. 5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more...

Ex: 416 nm. Em: 451 nm. Soluble in: DMSO. PacBlue succinimidyl ester is the same molecule to Pacific BlueT succinimidyl ester (Pacific BlueT is the trademark of ThermoFisher). The amine-reactive Pac Blue succinimidyl ester can be used to prepare the blue fluorescent antibody conjugates that can be well excited by...

Oregon Green(R) is a trademark of Life Technologies.

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. iFluor(TM) 594 dyes have fluorescence excitation and emission maxima of 590 nm and 610 nm respectively. iFluor(TM) 594 family has the spectral properties similar to those of Texas Red(R) and Alexa Fluor(R) 594 (Texas...

Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the...

Ex: 414 nm. Em: 508 nm. Soluble in: DMSO. AAT Bioquest's mFluorT dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluorT Violet 520 dyes have fluorescence excitation and...