Lyo Speedy NZY Direct Genotyping Kit

The Lyo Speedy NZY Direct Genotyping Kit features a lyophilized formulation of the Speedy Supreme NZYTaq 2x Master Mix, ensuring stable room temperature shipping. The performance of this kit is on par with its liquid-form counterpart, the Speedy NZY Direct Genotyping Kit (MB475). Engineered to process an array of tissue materials, including blood and non-invasive hair samples, these kits promise a seamless, animal-friendly genotyping experience. They integrate a streamlined one-tube sample preparation with a swift PCR amplification, enabling rapid lysis of biological samples, including mouse genotyping tissues samples (tail clipping and ear punch), in under 5 minutes. Lyo Speedy NZY Direct Genotyping Kit is validated for hair sample genotyping, which executed with minimal animal discomfort and without necessitating anaesthesia harmoniously combines ethical and efficient genotyping. The kit includes a potent Speed Lysis Enzyme Mix, meticulously developed for rapid sample lysis at room temperature. Post-lysis, a brief centrifugation secures the DNA-laden supernatant. Employing an aliquot of this lysate in tandem with the Lyo Speedy Supreme NZYTaq 2x Master Mix ensures lysis and PCR cycling times under one hour (5 min Lysis/Inactivation plus 44 min PCR cycle, total time 49 min). In addition, the master mix reconstitution buffer contains a blue dye for direct loading onto agarose gels, omitting the need for additional dye application. The Lyo Speedy NZY Direct Genotyping Kit also offers an alternative direct PCR protocol, facilitating PCR directly from undiluted, unpurified samples. The process produces ample template for numerous assays, compatible with a 96-well format, and assures sufficient template for a minimum of 20 genotyping PCR reactions. Finally, DNA fragments generated with the Lyo Speedy NZY Direct Genotyping Kit are 3'-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, DNA cloning and sequencing. Starting Material: Blood, Ear punch, Hair, Mouse tail, Skin. Features: Fast, Standard. Product Length: 0-1kb. Format: Lyophilized, Mastermix. Product Category: End-Point PCR. Protocol time: Approx. 45 min. Hot Start: Yes. Components: - Lyo Speedy Supreme NZYTaq 2x Master Mix, - Speedy Supreme NZYTaq 2x MM Reconstitution Buffer, - Speedy Lysis Buffer, - Speedy Lysis Enzyme Mix.