BPP Bioportide(TM) 10x Exploration Kit for Transformation of gram-positive bacteria

BPP Bioportides(TM) are novel multi-functional designer proteins that bind quickly, spontaneously, reversibly, and robustly nucleic acids and transport them across the host's cell membrane utilizing a specialized N-terminal cell-penetrating-peptide moiety. This domain is designed differently for each BPP Bioportide(TM), depending on which type of host cell is to be addressed. The BPP Bioportide(TM) 10x Exploration Kit for Transformation of gram-positive bacteria provides a ready-to-use system for efficient transformation of various microorganisms. The kit contains ten different BPP Bioportides(TM) that have been developed for specific membrane types with the aim of addressing organisms that have been difficult or impossible to transform until now. Successful gene transfer has been achieved in Anabaena, Nostoc, Synechocystis, Synechococcus, and Hydrogen-oxidizing bacteria to date. The BPP Bioportide(TM) enables the transport of nucleic acids, ranging from 22 nt to >10 kb, into cells. Both RNA and DNA, including single- and double-stranded, linear or circular forms, can be transferred reliably. This innovative reagent facilitates the uptake of nucleic acids without requiring inducing cell stress, i.e. heat shock, or competent cells, i.e. by means of electroporation or chemical treatment. The easy and fast protocol works with picogram to nanogram quantities of nucleic acids. BPP Bioportides(TM) show robust performance in extreme environments (up to 80°C, pH > 9.0, high salt concentrations).