Cloning and Expression Kits

The product of DNase I hydrolysis of single- or double-stranded DNA has a phosphate group at the 5' end and a hydroxyl group at the 3' end. DNase I activity is dependent on calcium ions and can be activated by magnesium ions or divalent manganese ions. In the presence of magnesium ions, DNase I can randomly cleave...

EntiLink(TM) PCR Master Mix is a ready-to-use conventional PCR master mix solution containing Taq DNA Polymerase, dNTP mix, MgCl2, and optimized buffer system. The amplification can be performed by adding primers and templates during the reaction, which greatly simplifies the operation steps of the experiment. The...

EnTurbo(TM)+ SYBR Color qPCR SuperMix is an optimized 2 x Real-time PCR premix containing HotStarTaq DNA Polymerase, SYBR Green I fluorochrome dye, dNTP and Mg2+ and High ROX. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing, to ensure a highly sensitive and specific...

Limitless(TM) ELZ Fushion Seamless Cloning Kit is a new, fast and efficient Gibson Assembly DNA directed cloning technology that allows multiple insertions of target genes at any position in any vector, eliminating the need for cloning. Dots and multiple cloning sites. The LimitlessTM ELZ Fushion Seamless Cloning...

The Lyo Speedy NZY Direct Genotyping Kit features a lyophilized formulation of the Speedy Supreme NZYTaq 2x Master Mix, ensuring stable room temperature shipping. The performance of this kit is on par with its liquid-form counterpart, the Speedy NZY Direct Genotyping Kit (MB475). Engineered to process an array of...

NZY-A PCR cloning kit was designed to allow the direct cloning of PCR products with 3'-A overhangs, which result from amplifications using non-proofreading DNA polymerases such as NZYTaq II DNA polymerase. The cloning vector was prepared by cutting NZYTech's pNZY28 with EcoR V and adding a 3' terminal thymidine at...

NZY-A Speedy PCR cloning kit was designed to carry out fast andefficient cloning of PCR products with 3'-A overhangs, which resultfrom amplifications using non-proofreading DNA polymerases suchas NZYTaq II DNA polymerase. This methodology combines theefficiency of an improved ligation buffer with the speed of...

NZY First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your...

NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit...

NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate...

NZY First-Strand cDNA Synthesis Flexible Pack is an optimized pack that includes the following components: the modified recombinant form of the Moloney Murine Leukemia Virus (MMuLV) Reverse Transcriptase (RNase H minus), which synthesizes the complementary DNA strand using either RNA or single-stranded DNA as...

The TCR Knockout Electroporation Kit is suitable for Jurkat cell line and primary T cell engineering using electroporation. The kit contains both the Cas9 enzyme (Streptococcus pyogenes) and the gRNAs targeting TRAC (T-Cell Receptor Alpha Constant) and TRBC (targets both T Cell Receptor Beta Constant 1 and T Cell...