Cloning and Expression Kits

NZY-blunt PCR cloning kit was designed to allow the direct cloning of PCR products with blunt ends, which result from PCR amplifications using proofreading DNA polymerases, into pNZY28. The pNZY28 vector provided was linearized at the EcoR V and dephosphorilated. Components: * Phos Buffer (10x) * Phos Enzyme Mix *...

NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated...

Biotium's proprietary fast-activating, chemically modified Cheetah HotStart Taq, suitable for fast qPCR, PEA assays, and other applications. Supplied as 500 U Taq (5 U/mL, 100 uL) with 10X dilution buffer and 25 mM MgCl2.

The PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps. Test Principle: A primer set homologous to the gene of interest and the vector is used to amplify a PCR product. This purified PCR product is incubated with restriction...

GenieClone DNA Assembly Cloning Kit allows for the highly efficient cloning and assembly of 1-5 DNA fragments in as little as 15 minutes into any vector at any site. This system utilizes ligation-independent technology and a novel GenieClone recombinase to significantly reduce self-ligating colonies. The enhanced...

GenieClone 5 Minute Ligation mix is a ready-to-use 2 x premix solution suitable for sticky-end ligation, blunt-end DNA ligation and TA ligation. The kit contains an optimised T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA...

In this kit, the plasmid DNA was purified rapidly by the modified SDS alkaline lysis combined with the method of selective adsorption of DNA on DNA preparation membrane. It applies to extract up to 20ug of high purity plasmid DNA from 1-5 ml bacterial culture. It has been used in molecular biology experiments such...

In this kit. Combining the method of preparing membrane with high adsorption amount of DNA and selectively adsorbing DNA to achieve the purpose of rapid purification of plasmid DNA. It applies to extract up to 60ug of high purity plasmid DNA from 5-30 ml bacterial culture. It has been used in molecular biology...

This kit uses a unique buffer system that combines with traditional isopropanol precipitation to quickly obtain large amounts of high-purity plasmid DNA. The plasmid DNA extracted using this kit can be applied to various routine operations. Including restriction enzyme digestion, PCR, sequencing, ligation,...

The kit recovers high-purity DNA fragments containing no salt or low salt and no impurities such as protein or RNA from the PCR reaction system. The 200 bp-10 kbp DNA fragment has a recovery rate of more than 80%. and can recover single-stranded,double-stranded DNA fragments and circular plasmid DNA. The recovered...

The kit is suitable for extracting high purity total DNA from fresh or frozen animal tissues, cells, blood, bacteria and other samples. DNA fragments with a maximum molecular weight of 50 KB can be purified without the use of toxic solvents such as phenol or chloroform and ethanol precipitation. The optimized buffer...

This product can rapidly separate and purify genomic DNA from 200-400 µl of fresh or frozen human or animal whole blood within 15-20 min. The product does not use proteinase K. After the whole blood is dissolved by Solution BFL1,the hemoglobin is removed by Solution BFL2 precipitation. The genomic DNA in the...