Zymogen granule protein 16 homolog B (ZG16B) (Active), Macaca fascicularis, recombinant

Organism: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey). Source: Mammalian cell. Expression Region: 23-169aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: GKMYGPGGGK YFTTTEDYDH EITGLRVSVG LLLVKSVQVK LGDTWDVKQG ASGGNTQEVT LQPGEYITKV FVAFQTFLRG MVLYTSKDRT FYFGKLDGQI FSVYPSQEGQ VLVGIYGQYG LLGIKSIGFE WNYPLEEPTT EPPVTVT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ZG16B at 2 µg/mL can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC50 is 20.15-28.98 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play an important role in the pathogenesis of pancreatic cancer. Reference: "Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques." Yan G., Zhang G., Fang X., Zhang Y., Li C., Ling F., Cooper D.N., Li Q., Li Y., van Gool A.J., Du H., Chen J., Chen R., Zhang P., Huang Z., Thompson J.R., Meng Y., Bai Y. Wang J. Nat. Biotechnol. 29:1019-1023(2011). Function: nan