Zinc Transporter ZIP5, Control Peptide (Solute Carrier Family 39 Member 5, Zrt- and Irt-like Protein

Control peptide for Z0510-07. Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, ZIP4, Zip4, Zip5, LIV-1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types. , Zip5 (SLC39A5) is similar in sequence to the Zip4 protein, which is involved in the uptake of dietary zinc. Co-expression of Zip4 and Zip5 in the intestine led to the hypothesis that these proteins play overlapping roles in the uptake of dietary zinc across the apical membrane of intestinal enterocytes. mZip5 localizes specifically to the basolateral membrane of polarized Madin-Darby canine kidney cells. Zip5 plays a central role in controlling organismal zinc status. Source: Synthetic peptide corresponding to 17aa of mouse ZIP5 (Accession #Q9D856), N-terminal, Extracellular domain. Molecular Weight: ~3kD, Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.