Zinc finger CCCH domain-containing protein 7B (ZC3H7B), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 481-915aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SYYLCKDMIN KQDCKYGDNC TFAYHQEEID VWTEERKGTL NRDLLFDPLG GVKRGSLTIA KLLKEHQGIF TFLCEICFDS KPRIISKGTK DSPSVCSNLA AKHSFYNNKC LVHIVRSTSL KYSKIRQFQE HFQFDVCRHE VRYGCLREDS CHFAHSFIEL KVWLLQQYSG MTHEDIVQES KKYWQQMEAH AGKASSSMGA PRTHGPSTFD LQMKFVCGQC WRNGQVVEPD KDLKYCSAKA RHCWTKERRV LLVMSKAKRK WVSVRPLPSI RNFPQQYDLC IHAQNGRKCQ YVGNCSFAHS PEERDMWTFM KENKILDMQQ TYDMWLKKHN PGKPGEGTPI SSREGEKQIQ MPTDYADIMM GYHCWLCGKN SNSKKQWQQH IQSEKHKEKV FTSDSDASGW AFRFPMGEFR LCDRLQKGKA CPDGDKCRCA HGQEE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 'ATA(A/T)' motif in the apical loop. Reference: "A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis." Treiber T., Treiber N., Plessmann U., Harlander S., Daiss J.L., Eichner N., Lehmann G., Schall K., Urlaub H., Meister G. Mol. Cell 66:270-284(2017). Function: nan