Zinc finger and BTB domain-containing protein 32 (ZBTB32), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-294aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSLPPIRLPS PYGSDRLVQL AARLRPALCD TLITVGSQEF PAHSLVLAGV SQQLGRRGQW ALGEGISPST FAQLLNFVYG ESVELQPGEL RPLQEAARAL GVQSLEEACW RARGDRAKKP DPGLKKHQEE PEKPSRNPER ELGDPGEKQK PEQVSRTGGR EQEMLHKHSP PRGRPEMAGA TQEAQQEQTR SKEKRLQAPV GQRGADGKHG VLTWLRENPG GSEESLRKLP GPLPPAGSLQ TSVTPRPSWA EAPWLVGGQP ALWSILLMPP RYGIPFYHST PTTGAWQEVW REQR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Reference: "Insights into strand exchange in BTB domain dimers from the crystal structures of FAZF and Miz1." Stogios P.J., Cuesta-Seijo J.A., Chen L., Pomroy N.C., Prive G.G. J. Mol. Biol. 400:983-997(2010). Function: nan