YTHDF2 (human recombinant)

YTH-Domain family member 2 (YTHDF2) is a reader protein that binds to N6-methyladenosine (m6A) to regulate mRNA degradation. YTHDF2 binds to m6A sites in the 3'-UTR of mRNA through its C-terminal YTH domain and recruits the CCR4-NOT deadenylase complex via its N-terminus to accelerate mRNA localization to decay sites, such as P-bodies, and promote subsequent degradation. Ythdf2 knockout results in a build-up of maternal mRNA in oocytes that induces female-specific infertility in mice. Conditional knockout of Ythdf2 increases the number of hematopoietic stem cells (HSCs) without shifting lineage differentiation or inducing malignancies. It also increases ex vivo expansion of human umbilical cord HSCs. This product is the C-terminus of YTHDF2 corresponding to residues 358-502 of the human full-length sequence.Synonyms: CAHL, CLL-associated Antigen KW-14, HGRG8, High-Glucose-Regulated Protein 8, Renal Carcinoma Antigen NY-REN-2, YTH Domain-containing Family Member 2, YTH N6-Methyladenosine RNA Binding Protein 2 (358-502, YTH domain). Purity: >90% estimated by SDS-PAGE. Source: Recombinant N-terminal GST-tagged YTHDF2 purified from E. coli. Amino Acids: 358-502. MW: 43.24 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 7.5, with 150 mM sodium chloride and 10% glycerol. UniProt Accession #: Q9Y5A9.