Xylosyltransferase 2 (XYLT2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 37-865aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GLEEDEAGEK GRQRKPRPLD PGEGSKDTDS SAGRRGSTGR RHGRWRGRAE SPGVPVAKVV RAVTSRQRAS RRVPPAPPPE APGRQNLSGA AAGEALVGAA GFPPHGDTGS VEGAPQPTDN GFTPKCEIVG KDALSALARA STKQCQQEIA NVVCLHQAGS LMPKAVPRHC QLTGKMSPGI QWDESQAQQP MDGPPVRIAY MLVVHGRAIR QLKRLLKAVY HEQHFFYIHV DKRSDYLHRE VVELAQGYDN VRVTPWRMVT IWGGASLLRM YLRSMRDLLE VPGWAWDFFI NLSATDYPTR TNEELVAFLS KNRDKNFLKS HGRDNSRFIK KQGLDRLFHE CDSHMWRLGE RQIPAGIVVD GGSDWFVLTR SFVEYVVYTD DPLVAQLRQF YTYTLLPAES FFHTVLENSL ACETLVDNNL RVTNWNRKLG CKCQYKHIVD WCGCSPNDFK PQDFLRLQQV SRPTFFARKF ESTVNQEVLE ILDFHLYGSY PPGTPALKAY WENTYDAADG PSGLSDVMLT AYTAFARLSL HHAATAAPPM GTPLCRFEPR GLPSSVHLYF YDDHFQGYLV TQAVQPSAQG PAETLEMWLM PQGSLKLLGR SDQASRLQSL EVGTDWDPKE RLFRNFGGLL GPLDEPVAVQ RWARGPNLTA TVVWIDPTYV VATSYDITVD TETEVTQYKP PLSRPLRPGP WTVRLLQFWE PLGETRFLVL PLTFNRKLPL RKDDASWLHA GPPHNEYMEQ SFQGLSSILN LPQPELAEEA AQRHTQLTGP ALEAWTDREL SSFWSVAGLC AIGPSPCPSL EPCRLTSWSS LSPDPKSELG PVKADGRLR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Reference: "Homozygosity for frameshift mutations in XYLT2 result in a spondylo-ocular syndrome with bone fragility, cataracts, and hearing defects." Munns C.F., Fahiminiya S., Poudel N., Munteanu M.C., Majewski J., Sillence D.O., Metcalf J.P., Biggin A., Glorieux F., Fassier F., Rauch F., Hinsdale M.E. Am. J. Hum. Genet. 96:971-978(2015). Function: nan