Xylanase 10B, Bacteroides ovatus

Xylanase 10B (BoXyn10B), assigned the E.C. number 3.2.1.8, is a derivative of Bacteroides ovatus. It is an endo-1,4-beta-xylanase that contains an extended substrate binding cleft that requires a high degree of occupancy for catalysis to occur. The recombinant BoXyn10B, purified from Escherichia coli, is a modular Glycoside Hydrolase family 10 (GH10) enzyme, with two GH10 catalytic module repeats and an internal family 22 Carbohydrate Binding Module (CBM22) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Xylanases. Family: GH10. Ec Number: 3.2.1.8. Organism: Bacteroides ovatus. Architecture: GH10-CBM22-GH10. Optimal pH: 7.2. Optimal Temperature: 37°C. Substrates: 1,4-beta-xylans