Wiskott-Aldrich syndrome protein (WAS), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 2-502aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SGGPMGGRPG GRGAPAVQQN IPSTLLQDHE NQRLFEMLGR KCLTLATAVV QLYLALPPGA EHWTKEHCGA VCFVKDNPQK SYFIRLYGLQ AGRLLWEQEL YSQLVYSTPT PFFHTFAGDD CQAGLNFADE DEAQAFRALV QEKIQKRNQR QSGDRRQLPP PPTPANEERR GGLPPLPLHP GGDQGGPPVG PLSLGLATVD IQNPDITSSR YRGLPAPGPS PADKKRSGKK KISKADIGAP SGFKHVSHVG WDPQNGFDVN NLDPDLRSLF SRAGISEAQL TDAETSKLIY DFIEDQGGLE AVRQEMRRQE PLPPPPPPSR GGNQLPRPPI VGGNKGRSGP LPPVPLGIAP PPPTPRGPPP PGRGGPPPPP PPATGRSGPL PPPPPGAGGP PMPPPPPPPP PPPSSGNGPA PPPLPPALVP AGGLAPGGGR GALLDQIRQG IQLNKTPGAP ESSALQPPPQ SSEGLVGALM HVMQKRSRAI HSSDEGEDQA GDEDEDDEWD D. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905). Important for efficient actin polymerization (PubMed:8625410, PubMed:12235133, PubMed:16275905). Possible regulator of lymphocyte and platelet function (PubMed:9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed:18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947). Reference: "Nuclear ARP2/3 drives DNA break clustering for homology-directed repair." Schrank B.R., Aparicio T., Li Y., Chang W., Chait B.T., Gundersen G.G., Gottesman M.E., Gautier J. Nature 559:61-66(2018). Function: nan