Wee1-like protein kinase (Wee1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-646aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSFLSRQQPP PTRRVGAAYS LRQKLIFSPG SDCEEEEEEE EEGSGHSTGE DSAFQEPDSP LPSARSPAEA EAERRRRSPG AEPSSPGELE DDLLLQGGGG GAQAAGGGAE GDSWEEEGFG SSSPVKSPST AYFLSSPFSP VRCGGPGDAS PQGCGAPRAM DDPCSPQPDY PSTPPHKTFR KLRLFDTPHT PKSLLSKARV IDSGSVKLRG SSLFMDTEKS GKREFDTRQT PQVNINPFTP DPVLLHSSGR CRGRKRAYFN DSSEDMEASD YEFEDETRPA KRITITESNM KSRYTTEFHE LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA VLGQHPHVVR YFSAWAEDDH MLIQNEYCNG GSLADAISEN YRVMSYLTEV ELKDLLLQVG RGLRYIHSMS LVHMDIKPSN IFISRTSIPN AVSEEGDEDD WISNKVMFKI GDLGHVTRIS SPQVEEGDSR FLANEVLQEN YSHLPKADIF ALALTVVCAA GAEPLPRNGE QWHEIRQGRL PRIPQVLSQE VTELLRVMIH PDPERRPSAM ELVKHSVLLS ASRKSAEQLR IELNAEKFKN SLLQKELKKA QMAAKVAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Reference: "Mouse Wee1 gene is repressed by Kruppel-like factor 3 (KLF3) via interaction with multiple upstream elements." Kitamura T., Suzuki H., Tamura T.A. Gene 492:361-367(2012). Function: nan