Vitamin K-dependent protein S (PROS1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 42-676aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: ANSLLEETKQ GNLERECIEE LCNKEEAREV FENDPETDYF YPKYLVCLRS FQTGLFTAAR QSTNAYPDLR SCVNAIPDQC SPLPCNEDGY MSCKDGKASF TCTCKPGWQG EKCEFDINEC KDPSNINGGC SQICDNTPGS YHCSCKNGFV MLSNKKDCKD VDECSLKPSI CGTAVCKNIP GDFECECPEG YRYNLKSKSC EDIDECSENM CAQLCVNYPG GYTCYCDGKK GFKLAQDQKS CEVVSVCLPL NLDTKYELLY LAEQFAGVVL YLKFRLPEIS RFSAEFDFRT YDSEGVILYA ESIDHSAWLL IALRGGKIEV QLKNEHTSKI TTGGDVINNG LWNMVSVEEL EHSISIKIAK EAVMDINKPG PLFKPENGLL ETKVYFAGFP RKVESELIKP INPRLDGCIR SWNLMKQGAS GIKEIIQEKQ NKHCLVTVEK GSYYPGSGIA QFHIDYNNVS SAEGWHVNVT LNIRPSTGTG VMLALVSGNN TVPFAVSLVD STSEKSQDIL LSVENTVIYR IQALSLCSDQ QSHLEFRVNR NNLELSTPLK IETISHEDLQ RQLAVLDKAM KAKVATYLGG LPDVPFSATP VNAFYNGCME VNINGVQLDL DEAISKHNDI RAHSCPSVWK KTKNS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Anticoagulant plasma protein, it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Reference: Human protein S cDNA encodes Phe-16 and Tyr 222 in consensus sequences for the post-translational processing.Ploos van Amstel H.K., van der Zanden A.L., Reitsma P.H., Bertina R.M.FEBS Lett. 222:186-190(1987). Function: Anticoagulant plasma protein, it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.