Uroplakin-1b (Upk1b), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 1-260aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MAKDDSTVRC FQGLLIFGHV IVGMCGIALT AECIFFVSDQ HSLYPLLEAT NNDDIFGAAW IGMFVGICLF CLSVLAIVGI MKSNRKILLA YFIMMFIVYG FEVASCITAA TQRDFFTTNL FLKQMLMRYQ NNSPPTNDDK WKNSYVTKTW DRLMLQDHCC GVNGPSDWQK YTSAFRVENS DADYPWPRQC CVMDKLQEPL NLDACKLGVP GYYHSQGCYE LISGPMDRHA WGVAWFGFAI LCWTFWVLLG TMFYWSRIEY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the asymmetric unit membrane (AUM), a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. Reference: n/a. Function: nan