Uromodulin (Umod), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 25-588aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: NSTEARRCSE CHNNATCTVD GVVTTCSCQT GFTGDGLVCE DMDECATPWT HNCSNSSCVN TPGSFKCSCQ DGFRLTPELS CTDVDECSEQ GLSNCHALAT CVNTEGDYLC VCPEGFTGDG WYCECSPGSC EPGLDCLPQG PDGKLVCQDP CNTYETLTEY WRSTEYGVGY SCDAGLHGWY RFTGQGGVRM AETCVPVLRC NTAAPMWLNG SHPSSSEGIV SRTACAHWSD QCCRWSTEIQ VKACPGGFYI YNLTAPPECN LAYCTDPSSV EGTCEECRVD EDCISDNGRW RCQCKQDSNI TDVSQLEYRL ECGANDIKMS LRKCQLQSLG FMNVFMYLND RQCSGFSESD ERDWMSIVTP ARNGPCGTVL RRNETHATYS NTLYLANAII IRDIIIRMNF ECSYPLDMKV SLKTSLQPMV SALNISLGGT GKFTVRMALF QSPTYTQPHQ GPSVMLSTEA FLYVGTMLDG GDLSRFVLLM TNCYATPSSN STDPVKYFII QDSCPRTEDT TIQVTENGES SQARFSVQMF RFAGNYDLVY LHCEVYLCDS TSEQCKPTCS GTRF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.By similarity Uromodulin, secreted form: In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Reference: "Tamm-Horsfall protein knockout mice are more prone to urinary tract infection: rapid communication."Bates J.M., Raffi H.M., Prasadan K., Mascarenhas R., Laszik Z., Maeda N., Hultgren S.J., Kumar S.Kidney Int. 65:791-797(2004). Function: Uromodulin