Unsaturated rhamnogalacturonyl hydrolase 105D, Bacteroides thetaiotaomicron

Unsaturated rhamnogalacturonyl hydrolase 105D (BtUrh105D), assigned the E.C. number 3.2.1.172, is a derivative of Bacteroides thetaiotaomicron. It catalyzes the hydrolysis of unsaturated rhamnogalacturonan disaccharide to yield unsaturated D-galacturonic acid and L-rhamnose. The recombinant BtUrh105D, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 105 (GH105) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Rhamnogalacturonases. Family: GH105. Ec Number: 3.2.1.172. Organism: Bacteroides thetaiotaomicron. Architecture: GH105. Optimal pH: 7.5. Optimal Temperature: 37°C. Substrates: unsaturated RGI oligosaccharides