Ulex europaeus (UEA I, Gorze, Furze)

Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000D and another of 32,000D. UEA I binds to many glycoproteins and glycolipids containing a -linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 50mM-100mM L-fucose, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.