UDP-glycosyltransferase 89C1 (UGT89C1), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: Yeast. Expression Region: 1-435aa. Protein Length: Full length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MTTTTTKKPH VLVIPFPQSG HMVPHLDLTH QILLRGATVT VLVTPKNSSY LDALRSLHSP EHFKTLILPF PSHPCIPSGV ESLQQLPLEA IVHMFDALSR LHDPLVDFLS RQPPSDLPDA ILGSSFLSPW INKVADAFSI KSISFLPINA HSISVMWAQE DRSFFNDLET ATTESYGLVI NSFYDLEPEF VETVKTRFLN HHRIWTVGPL LPFKAGVDRG GQSSIPPAKV SAWLDSCPED NSVVYVGFGS QIRLTAEQTA ALAAALEKSS VRFIWAVRDA AKKVNSSDNS VEEDVIPAGF EERVKEKGLV IRGWAPQTMI LEHRAVGSYL THLGWGSVLE GMVGGVMLLA WPMQADHFFN TTLIVDKLRA AVRVGENRDS VPDSDKLARI LAESAREDLP ERVTLMKLRE KAMEAIKEGG SSYKNLDELV AEMCL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Flavonol 7-O-rhamnosyltransferase that acts on kaempferol 3-O-glucoside and other 3-O-glycosylated flavonols like quercetin 3-O-glucoside, quercetin 3-O-galactoside and isorhamnetin 3-O-glucoside. Shows a strict specificity for UDP-rhamnose as donor and does not act on flavonol aglycones or 3-O-glycosylated anthocyanins. Reference: "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001). Function: Flavonol 7-O-rhamnosyltransferase that acts on kaempferol 3-O-glucoside and other 3-O-glycosylated flavonols like quercetin 3-O-glucoside, quercetin 3-O-galactoside and isorhamnetin 3-O-glucoside. Shows a strict specificity for UDP-rhamnose as donor and does not act on flavonol aglycones or 3-O-glycosylated anthocyanins.