UDP-glucuronosyltransferase 1A1 (UGT1A1), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 26-533aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: HAGKILLIPV DGSHWLSMLG AIQQLQQRGH EIVVLAPDAS LYIRDGAFYT LKTYPVPFQR EDVKESFVSL GHNVFENDSF LQRVIKTYKK IKKDSAMLLS GCSHLLHNKE LMASLAESSF DVMLTDPFLP CSPIVAQYLS LPTVFFLHAL PCSLEFEATQ CPNPFSYVPR PLSSHSDHMT FLQRVKNMLI AFSQNFLCDV VYSPYATLAS EFLQREVTVQ DLLSSASVWL FRSDFVKDYP RPIMPNMVFV GGINCLHQNP LSQEFEAYIN ASGEHGIVVF SLGSMVSEIP EKKAMAIADA LGKIPQTVLW RYTGTRPSNL ANNTILVKWL PQNDLLGHPM TRAFITHAGS HGVYESICNG VPMVMMPLFG DQMDNAKRME TKGAGVTLNV LEMTSEDLEN ALKAVINDKS YKENIMRLSS LHKDRPVEPL DLAVFWVEFV MRHKGAPHLR PAAHDLTWYQ YHSLDVIGFL LAVVLTVAFI TFKCCAYGYR KCLGKKGRVK KAHKSKTH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4-methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1. Reference: Thirteen UDP-glucuronosyltransferase genes are encoded at the human UGT1 gene complex locus.Gong Q.H., Cho J.W., Huang T., Potter C., Gholami N., Basu N.K., Kubota S., Carvalho S., Pennington M.W., Owens I.S., Popescu N.C.Pharmacogenetics 11:357-368(2001). Function: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4-methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.